Description

Product Description

Azacitidine is a chemically synthesized nucleoside analogue designed to inhibit DNA methyltransferase enzymes, thereby preventing aberrant DNA methylation and allowing reactivation of silenced genes in preclinical research models. Its molecular structure incorporates a cytidine base modified at the 5-position, which is essential for covalent binding to DNMTs and subsequent hypomethylation of DNA.

In vitro studies demonstrate that Azacitidine incorporates into DNA and RNA during replication and transcription, resulting in inhibition of methylation and alterations in gene expression patterns. Preclinical tumor models have shown that Azacitidine treatment leads to cell cycle arrest, apoptosis induction, and suppression of proliferation in various cancer cell lines.

Azacitidine is highly soluble in aqueous buffers and DMSO, facilitating use in in vitro assays and experimental cell culture studies. It is also stable under recommended laboratory storage conditions, with a verified purity of ≥99%, ensuring reproducibility across experiments.

Experimental applications of Azacitidine include:

• Epigenetic studies: Investigating DNA hypomethylation and reactivation of silenced tumor suppressor genes.

• Cancer biology research: Studying cell cycle regulation, apoptosis pathways, and tumor suppression mechanisms.

• Combination therapy assessments: Evaluating synergistic effects with chemotherapeutics, immunomodulators, or other epigenetic modulators.

• Gene expression profiling: Using genome-wide methylation and transcriptional analysis to assess epigenetic changes.

• Mechanistic research: Exploring DNMT inhibition kinetics, cellular uptake, and dose-response relationships.

Azacitidine’s high selectivity, metabolic stability, and reproducible in vitro incorporation make it a valuable tool for laboratory research in epigenetics, oncology, and preclinical pharmacology.

Product Specifications

Mechanism of Action

Azacitidine functions primarily as a DNA methyltransferase inhibitor by incorporating into DNA and RNA, forming covalent complexes with DNMT enzymes. This leads to the hypomethylation of DNA and reactivation of silenced genes, particularly tumor suppressor genes, enabling detailed preclinical studies of epigenetic regulation.

DNMT Inhibition:
Azacitidine binds covalently to DNMTs during DNA replication, preventing methylation of cytosine residues in CpG islands. This inhibition allows previously silenced genes to be transcribed, facilitating the study of epigenetic mechanisms and gene expression modulation.

Gene Reactivation:
Through hypomethylation, Azacitidine reactivates tumor suppressor genes and regulatory RNAs, enabling investigations into the functional consequences of epigenetic changes on tumor growth, cell cycle progression, and apoptosis.

Downstream Effects:
By modulating methylation patterns, Azacitidine impacts cellular proliferation, apoptosis induction, and differentiation pathways. Researchers employ Azacitidine to explore global methylation changes, epigenome editing, and therapeutic targets in preclinical models.

Experimental Applications:

• In vitro DNA methylation assays and RNA transcription studies

• Preclinical tumor models for epigenetic therapy research

• Combination studies with chemotherapeutic or immunomodulatory agents

• High-throughput epigenetic screenings

  

Side Effects

In laboratory studies, Azacitidine shows effects consistent with DNMT inhibition:

• Cell cycle arrest: Typically at S-phase due to incorporation into DNA.

• Apoptosis induction: Activation of intrinsic and extrinsic pathways in tumor cells.

• Gene expression modulation: Reactivation of silenced genes and non-coding RNAs.

• Cytotoxicity: Dose-dependent effects observed in high-concentration in vitro studies.

Laboratory Safety Guidelines:

• Handle with gloves, lab coat, and eye protection.

• Use in a fume hood to avoid inhalation.

• Avoid direct skin contact and ingestion.

• Dispose of waste according to institutional chemical safety protocols.

Azacitidine is intended strictly for laboratory research and is not approved for human or veterinary use.

Usage Instructions

• Dissolve in sterile water or DMSO for in vitro studies; typical concentrations range from 1–10 μM depending on experimental design.

• Store reconstituted solutions at –80 °C for short-term use, and freeze-dried powder at 2–8 °C protected from light for up to 24 months.

• Perform all experiments following institutional biosafety guidelines.

Application Cases

• Case 1: Epigenetic reactivation of tumor suppressor genes in leukemia cell lines using Azacitidine, assessing cell cycle arrest and apoptosis markers.

• Case 2: Combination therapy study with Azacitidine and histone deacetylase inhibitors to evaluate synergistic effects on gene expression and tumor cell viability.

• Case 3: Preclinical tumor xenograft study measuring the effect of Azacitidine on DNA methylation patterns and tumor growth inhibition.

Shipping Guarantee

All shipments of Azacitidine are handled using validated cold-chain logistics to preserve compound integrity. Each package is sealed in moisture-proof containers with secondary protective wrapping and continuous temperature monitoring. Products are shipped via express international couriers with tracking and insurance. Customers receive real-time updates on shipment status to ensure timely and secure delivery.

Trade Assurance

We guarantee the authenticity and ≥99% purity of Azacitidine in compliance with HPLC, MS, and NMR analytical standards. Each batch is accompanied by a Certificate of Analysis (CoA). Our trade assurance policy ensures replacement or full refund in the unlikely event of deviation from the listed specifications. We adhere strictly to quality management systems to provide reliable research-grade chemicals.

Payment Support

We accept flexible and secure global payment methods including PayPal, major credit cards (Visa, MasterCard, American Express), telegraphic transfer (T/T), and cryptocurrencies (USDT, Bitcoin, Ethereum). All transactions are encrypted and verified to ensure confidentiality and fund security. Invoices and payment confirmations are provided for every order.

Keywords

Azacitidine, DNA methyltransferase inhibitor, DNMT inhibitor, nucleoside analogue, epigenetic research, tumor suppression studies, gene reactivation, preclinical oncology research, small-molecule epigenetic modulator, laboratory research chemical, research chemical supplier, high-purity Azacitidine

Disclaimer

All products listed are intended solely for laboratory research use. They are not drugs, medical devices, or diagnostics and should not be administered to humans or animals. Researchers must handle all materials according to institutional biosafety and chemical safety guidelines. The information provided is for scientific reference only and does not imply therapeutic efficacy, safety, or regulatory approval.