Description

Product Description

Oxytocin (CAS 50-56-6) is a highly characterized peptide extensively used in molecular biology, biochemical assays, structural modeling, and receptor interaction studies. Produced via solid-phase peptide synthesis and purified through advanced chromatographic methods, this high-purity Oxytocin peptide offers exceptional consistency and stability. Supplied as a lyophilized powder, it maintains structural integrity for long-term storage and flexible experimental use.

In research settings, Oxytocin is utilized to study ligand–receptor interactions, signaling pathways, peptide conformational dynamics, and molecular recognition events. Its well-defined disulfide-bonded cyclic structure and high solubility allow reliable reproducibility across analytical workflows, including HPLC, mass spectrometry, spectroscopy, and computational modeling. The peptide’s predictable behavior supports comparative studies, peptide library validation, and analytical calibration.

This high-purity Oxytocin is suitable for in vitro biochemical experiments such as receptor-binding assays, peptide–protein interaction modeling, structural conformation studies, and functional mapping. Its reproducibility ensures consistent assay outcomes and reliable reference standards. The lyophilized format allows precise reconstitution and controlled concentration preparation, making it ideal for laboratory protocols requiring strict experimental consistency.

Researchers may also use Oxytocin as a model ligand for evaluating G-protein coupled receptor interactions, exploring secondary messenger dynamics, and mapping receptor selectivity. Its chemical stability and defined sequence make it appropriate for molecular docking, SAR analysis, and multi-omics integration studies. Bulk packaging and factory-direct supply make it a convenient option for laboratories performing high-throughput peptide research.

Oxytocin is intended exclusively for laboratory research and should be handled according to standard safety practices. It is not for human or veterinary use, and all experimental applications must remain within controlled in vitro or analytical research environments.

Product Specifications

Solubility and Concentration Recommendations

• Prepare fresh working solutions using sterile water or compatible buffers.

• Gently mix; avoid vortexing that may induce aggregation.

• Use low-binding consumables to maintain accurate peptide concentrations.

  

Mechanism of Action

Oxytocin (CAS 50-56-6) functions as a well-characterized peptide ligand for in vitro receptor-binding, signaling, and structural studies. Its cyclic disulfide-bonded structure confers high stability and receptor selectivity, making it an ideal tool for laboratories investigating peptide–receptor interactions, conformational dynamics, and downstream biochemical pathways in controlled experimental models.

Receptor Binding and Affinity

Oxytocin engages the oxytocin receptor (OTR) analogs in laboratory assays, facilitating high-precision studies of ligand–receptor affinity and structural selectivity. The peptide’s cyclic conformation promotes consistent and reproducible binding events, which are essential for modeling receptor–ligand interactions, evaluating structure–activity relationships, and validating computational docking simulations. Researchers often employ Oxytocin in in vitro assays to compare binding kinetics among peptide analogs or to benchmark receptor activation models.

Signal Transduction and Secondary Messenger Investigation

Although used strictly in research settings, Oxytocin provides a reliable model to study molecular signaling mechanisms. Its interaction with receptor analogs can be leveraged to explore in vitro secondary messenger dynamics, such as cyclic AMP modulation, protein phosphorylation patterns, and downstream effector activity within controlled assay frameworks. These investigations enable the characterization of peptide-mediated pathway modulation and temporal response patterns under defined experimental conditions.

Conformational and Structural Dynamics

The cyclic disulfide-bonded structure of Oxytocin supports advanced structural analyses, including:

• Nuclear Magnetic Resonance (NMR) spectroscopy

• Circular Dichroism (CD) spectroscopy

• Fourier-Transform Infrared (FTIR) spectroscopy

• Computational peptide folding and docking simulations

These studies provide insights into peptide folding, stability, receptor interface geometry, and conformational flexibility, allowing researchers to understand structure–function relationships and peptide–protein interactions.

Analytical and Biophysical Research Applications

Oxytocin is widely used as a high-purity peptide standard in analytical and biophysical research. Applications include:

• Method development for HPLC and LC-MS

• Benchmarking peptide quantification assays

• Investigating peptide-surface or peptide-material interactions in controlled laboratory setups

• Validating reproducibility and sensitivity of biochemical detection methods

Summary

Overall, Oxytocin’s mechanism of action in research focuses on controlled receptor engagement, predictable peptide folding, and consistent biochemical signaling in in vitro models. Its structural stability, reproducible binding characteristics, and well-defined cyclic conformation make it a versatile tool for laboratories studying peptide–receptor interactions, molecular modeling, analytical method development, and peptide structural dynamics. High-purity, factory-manufactured Oxytocin provides reliable performance for laboratories seeking standardized reagents for advanced molecular and biophysical research workflows.

Applications

Oxytocin (CAS 50-56-6) is a versatile research peptide widely utilized in in vitro molecular biology, analytical, structural, and biochemical studies. Its high purity, defined cyclic structure, and reproducible behavior make it ideal for laboratories investigating peptide–receptor interactions, signaling dynamics, structural conformation, and method development.

1. Receptor-Binding and Molecular Signaling Studies

• Serves as a model ligand for oxytocin receptor analogs in controlled in vitro assays.

• Facilitates studies on receptor-ligand affinity, conformational selectivity, and binding kinetics.

• Supports analysis of downstream signaling pathways, including secondary messenger dynamics and phosphorylation patterns in molecular research workflows.

2. Structural Biology and Peptide Conformation Research

• Ideal for NMR, CD spectroscopy, and FTIR experiments to explore peptide folding, cyclic structure stability, and disulfide-bonded conformation.

• Enables computational modeling and molecular docking studies to examine receptor interface geometry, peptide folding, and ligand-receptor interaction mechanisms.

• Provides a reference standard for comparative analysis of peptide analogs and structure-activity relationship (SAR) studies.

3. Analytical Method Development and Biochemical Assays

• Used as a calibration and reference peptide in HPLC, LC-MS, and spectroscopy-based analytical workflows.

• Supports validation of assay sensitivity, accuracy, linearity, and reproducibility across laboratory experiments.

• Allows reliable benchmarking of peptide quantification protocols, surface-binding assays, and material interaction studies.

4. Biophysical and Laboratory Modeling Applications

• Applicable in peptide-surface interaction studies, adsorption profiling, and interface characterization.

• Serves as a standardized peptide for method optimization, assay reproducibility, and comparative laboratory evaluations.

• Useful for high-throughput screening of peptide libraries and evaluating ligand selectivity in controlled molecular systems.

5. Multi-Omic and Computational Integration

• Facilitates integration into proteomics workflows, signaling network simulations, and computational modeling pipelines.

• Acts as a model peptide in systems biology studies to understand receptor-mediated molecular interactions.

• Enables cross-platform data comparison, linking structural, analytical, and biochemical results for reproducible research outcomes.

Summary:
High-purity Oxytocin supports diverse research applications, ranging from receptor-binding and signaling studies to analytical method development, structural conformation analysis, and computational modeling. Its consistent performance and defined cyclic structure make it a dependable tool for laboratories conducting advanced molecular, biochemical, and multi-omic investigations.

Research Models

Oxytocin (CAS 50-56-6) is widely applied in in vitro molecular, biochemical, structural, and analytical research models. Its high purity, defined cyclic structure, and reproducible properties make it an ideal tool for laboratories seeking precise, reliable, and controllable peptide behavior in experimental settings. The following research model categories illustrate its versatility and practical use.

1. Receptor-Ligand Binding Models

• Oxytocin serves as a model ligand for oxytocin receptor analogs in in vitro receptor-binding studies.

• Facilitates measurement of binding affinity, kinetics, and receptor conformational dynamics.

• Supports comparative studies of peptide analogs and ligand selectivity assessments.

2. Intracellular Signaling Models

• Used to study secondary messenger dynamics and downstream pathway regulation in controlled biochemical assays.

• Allows investigation of cyclic AMP modulation, protein phosphorylation patterns, and effector activation in laboratory settings.

• Enables detailed characterization of signaling pathways and temporal response profiling for mechanistic research.

3. Structural and Conformational Models

• Employed in NMR, CD spectroscopy, FTIR, and computational modeling to examine peptide folding, stability, and cyclic disulfide conformation.

• Supports peptide structure-function relationship studies and molecular docking simulations with receptor analogs.

• Provides a reference standard for analyzing structural stability of peptide libraries and analogs.

4. Analytical Method Development Models

• Oxytocin is a high-purity standard for HPLC, LC-MS, and spectroscopy-based method validation.

• Used to benchmark assay sensitivity, linearity, and reproducibility.

• Serves in surface-binding studies, peptide quantification validation, and analytical workflow optimization.

5. Biophysical and Interaction Models

• Enables controlled investigation of peptide-surface interactions, adsorption kinetics, and interface characterization.

• Useful for evaluating peptide-material interactions and biophysical assay performance.

• Facilitates comparative evaluation of peptide binding across substrates or experimental platforms.

6. Computational and Multi-Omic Integration Models

• Compatible with molecular docking, receptor modeling, and in silico peptide-receptor interaction studies.

• Supports integration into proteomics workflows, signaling network simulations, and systems biology studies.

• Provides a reproducible reference for cross-platform comparisons and modeling of peptide-mediated molecular interactions.

Summary:
High-purity Oxytocin offers a flexible platform for controlled in vitro research, supporting receptor-binding studies, signaling pathway analysis, structural conformation evaluation, analytical method development, and computational modeling. Its predictable structure, receptor-binding consistency, and chemical stability make it a dependable peptide for laboratories conducting advanced molecular and biochemical research.

Experimental Design Considerations

When using Oxytocin (CAS 50-56-6) in research, careful planning of experimental design is essential to ensure reproducibility, data accuracy, and safe laboratory handling. The following guidelines focus exclusively on in vitro, analytical, and structural research models, in line with controlled laboratory standards.

1. Define Experimental Objectives and Endpoints

• Clearly identify primary and secondary research goals, such as receptor-binding affinity, secondary messenger dynamics, structural stability, or analytical assay validation.

• Match assay formats to endpoints: binding studies for receptor kinetics, spectroscopy for conformational analysis, or LC-MS/HPLC for quantification.

• Establish expected dynamic ranges and limits of detection prior to experimentation.

2. Peptide Concentration and Titration

• Conduct preliminary titration experiments to determine the linear response range of the assay.

• Typical working concentrations range from 0.1–5 mg/mL depending on the assay and detection method.

• Prepare serial dilutions in compatible buffers; avoid extreme pH or high organic solvent concentrations unless specifically required.

3. Controls and Replication

• Include negative controls (buffer only) and positive/benchmark controls (validated Oxytocin reference peptide) to ensure assay reliability.

• Implement technical replicates (≥3) to improve precision and reproducibility.

• Where applicable, replicate experiments under identical conditions to evaluate variability.

4. Reconstitution and Handling

• Reconstitute lyophilized Oxytocin gently in sterile water or compatible buffers; avoid vigorous vortexing that may cause aggregation.

• Aliquot reconstituted solutions into single-use volumes to minimize repeated freeze-thaw cycles.

• Use low-binding consumables to prevent peptide loss during preparation.

5. Buffer and Matrix Considerations

• Use buffers that maintain structural integrity (e.g., neutral pH such as PBS or HEPES).

• Assess matrix effects that could influence binding, retention time, or detection in analytical assays.

• Validate buffer compatibility for downstream measurements such as spectroscopy or chromatography.

6. Analytical Validation and Calibration

• Validate all methods (HPLC, LC-MS, spectroscopic assays) for linearity, precision, accuracy, and recovery.

• Use internal standards or reference peptides to correct for instrument or sample variability.

• Document retention times, fragmentation patterns, and calibration curves for traceability.

7. Data Documentation and Reproducibility

• Record all experimental parameters: lot number, COA reference, peptide concentration, buffer composition, and instrument settings.

• Maintain detailed laboratory notebooks or digital records to facilitate replication and verification.

• Follow version-controlled SOPs for peptide handling, assay execution, and data analysis.

8. Troubleshooting and Optimization

• Low signal: verify peptide integrity via HPLC/MS, reduce adsorption losses using low-binding materials.

• High background: check buffer composition, solvent purity, and possible carryover.

• Aggregation: optimize solvent, reduce peptide concentration, and apply gentle mixing techniques.

9. Safety and Compliance

• Handle Oxytocin exclusively in laboratory environments with appropriate personal protective equipment.

• Adhere to institutional safety protocols and chemical waste disposal regulations.

• Material is for research use only and not intended for human or veterinary applications.

Summary:
Adhering to these experimental design principles ensures that Oxytocin provides consistent, reproducible results in receptor-binding studies, signaling pathway analysis, structural evaluations, and analytical method development. Careful attention to concentration, controls, buffer compatibility, and documentation maximizes data quality while maintaining safe laboratory practices.

Laboratory Safety & Handling Guidelines

• Handle Oxytocin in a controlled lab environment with gloves, lab coat, and eye protection

• Avoid inhalation or direct contact with powder

• Store lyophilized peptide at -20°C, protected from light and moisture

• Use low-disturbance techniques during reconstitution and preparation

• Dispose of residues according to institutional safety and chemical waste protocols

• Material is for research use only and must not be used in humans or animals

  

Integration with Multi-Omic & Computational Studies

Oxytocin (CAS 50-56-6) is a high-purity peptide that can be seamlessly integrated into multi-omic and computational research workflows, providing a versatile platform for mechanistic studies, structural modeling, and systems biology investigations. Its defined cyclic structure, predictable receptor-binding properties, and stable biochemical profile make it an ideal reference peptide for laboratories exploring complex molecular interactions and signaling networks.

1. Proteomics and Signaling Network Integration

• Oxytocin can be used as a standard in proteomic assays to assess peptide–protein interactions, receptor engagement, and post-translational modifications.

• Incorporation into signaling pathway models allows for in vitro validation of peptide-mediated molecular events.

• Supports comparative evaluation across peptide analogs, enabling researchers to map structural features to functional outcomes.

2. Computational Modeling and Molecular Docking

• Serves as a reliable ligand for in silico receptor modeling and molecular docking studies.

• Researchers can simulate Oxytocin–receptor interactions to predict binding affinities, conformational changes, and potential structural modifications.

• Supports high-throughput computational screening for peptide analogs, providing a benchmark for virtual SAR (structure–activity relationship) studies.

3. Multi-Omic Data Integration

• Oxytocin can be included in multi-omic workflows, such as proteomics, transcriptomics, and metabolomics, to examine peptide-mediated network responses under controlled laboratory conditions.

• Enables correlation of peptide structural features with functional outcomes across datasets, enhancing mechanistic understanding of signaling pathways.

• Facilitates cross-platform validation, linking in vitro observations with computational predictions to improve reproducibility and interpretability.

4. Systems Biology and Pathway Mapping

• Provides a standardized tool for mapping receptor-mediated signaling networks in molecular simulations.

• Can be used to model temporal dynamics of peptide-receptor interactions and downstream biochemical events.

• Supports integration of experimental data into computational frameworks to predict pathway modulation, aiding hypothesis generation in laboratory research.

5. Analytical and Structural Computational Studies

• Oxytocin’s defined sequence and cyclic conformation allow for benchmarking of structural prediction algorithms, folding simulations, and peptide–protein interaction models.

• Facilitates method validation for computational workflows such as docking, energy minimization, and structural alignment.

• Can serve as a reference standard for validating emerging computational pipelines in peptide research.

Summary:
High-purity Oxytocin is a versatile tool for integrating in vitro research with computational and multi-omic studies. Its stable structure, reproducible receptor interactions, and defined biochemical properties allow laboratories to bridge experimental assays with predictive modeling, systems biology analysis, and multi-layered omic data integration, enhancing mechanistic insight and data reproducibility.

Keywords

Oxytocin, Oxytocin peptide, CAS 50-56-6, high-purity peptide, freeze-dried peptide, wholesale peptide, laboratory-grade peptide, receptor-binding peptide, analytical peptide standard, peptide structural research

Shipping Guarantee

• Temperature-controlled delivery preserves peptide stability during transit.

• Tamper-proof packaging ensures safe and intact arrival.

• Global logistics coverage guarantees reliable delivery to research laboratories worldwide.

• Bulk packaging options available for large-scale research applications.

Trade Assurance

• Factory-direct sourcing ensures authenticity and consistent high purity.

• Verified COA documents accompany every batch for transparency.

• Stable supply chain supports continuous laboratory research workflows.

• OEM customization available for institutional or commercial research purposes.

Payment Support

• Accepts bank transfer, PayPal, and major credit cards for secure transactions.

• Supports corporate procurement channels for bulk or repeated orders.

• Flexible payment methods facilitate seamless international and institutional purchasing.

Disclaimer

Oxytocin is intended exclusively for laboratory research purposes.
It is not for human or veterinary use, and no clinical or therapeutic applications are implied.
Researchers must follow all institutional safety protocols when handling this material.

References

1. PubChem – Oxytocin
   https://pubchem.ncbi.nlm.nih.gov/compound/Oxytocin

2. NCBI Protein Database – Oxytocin
   https://www.ncbi.nlm.nih.gov/protein/

3. RCSB Protein Data Bank (PDB) – Oxytocin-Receptor Structures
   https://www.rcsb.org/

4. ScienceDirect – Analytical Peptide Methods
   https://www.sciencedirect.com/

5. Nature Protocols – Peptide Structural Analysis
   https://www.nature.com/nprot/

6. Analytical Chemistry – Peptide Calibration Studies
   https://pubs.acs.org/journal/ancham

7. Journal of Peptide Science – Structural and Functional Studies
   https://onlinelibrary.wiley.com/journal/10991308

8. Frontiers in Molecular Biosciences – GPCR-Peptide Interactions
   https://www.frontiersin.org/journals/molecular-biosciences

9. Journal of Chromatography B – Peptide Analytical Method Development
   https://www.sciencedirect.com/journal/journal-of-chromatography-b

10. Protein & Peptide Letters – Analog Research
    https://www.eurekaselect.com/journal/28