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Calcium Folinate | CAS 1492-18-8 | 100 mg | Preclinical Folate Metabolism and Chemosensitization Research
Original price was: $3.00.$2.00Current price is: $2.00.
Calcium Folinate (CAS 1492-18-8) is a high-purity folinic acid derivative supplied as 100 mg research-grade powder. It is widely used in preclinical studies for folate metabolism, thymidylate synthase modulation, chemosensitization, DNA synthesis, and cytotoxicity research. This laboratory reagent is ideal for mechanistic investigations and combination therapy modeling.
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Product Description
Calcium Folinate (Leucovorin, CAS 1492-18-8) is the calcium salt of 5-formyl tetrahydrofolate, a biologically active folate derivative. It is widely employed in preclinical and laboratory research to study folate-mediated one-carbon metabolism, thymidylate synthase regulation, DNA synthesis, and chemosensitization mechanisms.
In preclinical oncology research, Calcium Folinate is often used to modulate the activity of chemotherapeutic agents, such as fluoropyrimidines, by stabilizing ternary complexes with thymidylate synthase, enhancing drug efficacy, and reducing off-target toxicity. Its powder form (100 mg) provides flexibility in preparing precise concentrations for in vitro or in vivo studies, supporting detailed mechanistic investigation.
Mechanistically, Calcium Folinate enters cells via folate transporters and is converted to 5,10-methylene-tetrahydrofolate (CH2-THF), serving as a cofactor for thymidylate synthase and purine synthesis. This facilitates DNA replication and repair and is essential for studies exploring DNA synthesis inhibition, cell-cycle progression, and apoptotic responses under chemotherapeutic challenge.
Researchers employ Calcium Folinate powder for combination studies with 5-fluorouracil, methotrexate, or other antifolate agents to investigate synergistic cytotoxic effects, chemosensitization pathways, and resistance mechanisms. Its high-purity laboratory-grade powder ensures reproducibility, stability, and compatibility with cell culture, xenograft, and pharmacokinetic research models.
Calcium Folinate also serves as a tool to evaluate folate-mediated metabolic flux, one-carbon transfer reactions, methylation status, and nucleotide synthesis under experimental conditions. Bulk quantities and precise 100 mg packaging facilitate extended preclinical studies, high-throughput assays, and combination therapy modeling.
Product Specifications
| Parameter | Specification / Data |
|---|---|
| Chemical Name / Synonyms | Calcium Folinate; Leucovorin Calcium; 5-formyl tetrahydrofolate calcium; C20H23CaN7O7 |
| CAS Number | 1492-18-8 |
| Molecular Formula | C20H23CaN7O7 |
| Molecular Weight | 473.45 g/mol |
| Purity / Assay | ≥99% |
| Appearance | White to off-white crystalline powder |
| Dosage Form | 100 mg powder for research |
| Solubility | Soluble in water; slightly soluble in ethanol |
| Storage Temperature | 2–8 °C, protect from light and moisture |
| Stability | Stable under refrigeration; avoid repeated freeze-thaw |
| Mechanistic Target | Thymidylate synthase, folate metabolism, DNA synthesis |
| Structural Category | Folinic acid derivative, cofactor in one-carbon metabolism |
| Applications | DNA synthesis research, thymidylate synthase modulation, chemosensitization studies, cell-cycle analysis, combination therapy evaluation, drug resistance modeling |
| Analytical Methods | HPLC, MS, NMR verified; batch consistency confirmed |
| Regulatory Notes | For laboratory research use only |
| Origin | Factory chemical supplier, China; OEM & bulk production available |
| Additional Data | SMILES: C1C(C(C(C(O1)CO)N2C=NC3=C2N=CN=C3N)O)O.Ca; CoA provided for each batch |
Mechanism of Action
Folate Metabolism and DNA Synthesis
Calcium Folinate is metabolized intracellularly to 5,10-methylene-THF (CH2-THF), which acts as a cofactor for thymidylate synthase (TS). CH2-THF donation is critical for converting deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), facilitating DNA synthesis and repair.
Thymidylate Synthase Modulation
Stabilizes ternary complex with 5-fluorouracil and TS
Enhances inhibition of TS activity in preclinical studies
Reduces off-target toxicity in combination with antifolate chemotherapy
Chemosensitization Pathways
Increases cytotoxicity of fluoropyrimidine-based drugs
Supports combination studies with methotrexate or pemetrexed
Enables evaluation of resistance mechanisms linked to folate transporter activity or TS expression
Cell-Cycle and Apoptosis Impact
Promotes S-phase progression in normal folate metabolism
Modulates DNA synthesis checkpoint signaling
Impacts apoptotic response in combination therapy models
Applications
Preclinical folate metabolism research
Thymidylate synthase inhibition studies
Chemosensitization and combination therapy modeling
DNA synthesis and replication studies
Apoptosis and cytotoxicity evaluation
Cell-cycle and checkpoint analysis
Resistance mechanism investigation
High-throughput in vitro screening
In vivo xenograft studies for chemotherapeutic research
Side Effects (Preclinical Observations)
Cellular Effects:
Altered DNA synthesis under folate modulation
S-phase checkpoint activation
Cytotoxicity enhancement in combination with antifolate agents
Apoptotic Effects:
Caspase-dependent apoptosis observed under chemotherapeutic challenge
Mitochondrial stress induced when combined with DNA synthesis inhibitors
Laboratory Handling:
Handle using PPE and fume hood
Avoid ingestion, inhalation, or skin contact
Strictly for laboratory research use
Keywords
Calcium Folinate, Leucovorin Calcium, CAS 1492-18-8, 100 mg powder, folinic acid derivative, thymidylate synthase modulator, folate metabolism research, chemosensitization studies, DNA synthesis inhibitor, combination therapy research, cytotoxicity assay reagent, high-purity research chemical, preclinical oncology reagent, laboratory research reagent, OEM & bulk supply.
Shipping Guarantee
All shipments of Calcium Folinate 100 mg powder are securely packaged, protected from moisture and light, and shipped via tracked international couriers. Cold-chain shipping is used where necessary to maintain compound stability and integrity.
Trade Assurance
≥99% purity verified by HPLC, MS, NMR
Batch consistency validated
Certificate of Analysis (CoA) provided
OEM & bulk production available
Replacement or refund guaranteed for deviation from listed specifications
Payment Support
PayPal
Credit Cards (Visa, MasterCard, AmEx)
Bank Transfer (T/T)
USDT / BTC / ETH
Corporate invoicing for bulk orders
Disclaimer
Calcium Folinate is supplied for laboratory research only. Not for human or veterinary use. Researchers must follow safety guidelines. Supplier assumes no liability for misuse.
References
Additional information
| Weight | 1.1 kg |
|---|---|
| Dimensions | 18 × 16 × 18 cm |
1. What is Calcium Folinate used for in preclinical research?
It is used to study folate metabolism, thymidylate synthase modulation, DNA synthesis, and chemosensitization mechanisms.
2. What is the purity of Calcium Folinate supplied?
≥99% verified using HPLC, MS, and NMR.
3. How should Calcium Folinate powder be stored?
Store at 2–8 °C, protect from light and moisture. Avoid repeated freeze-thaw cycles.
4. Can Calcium Folinate be used in combination therapy studies?
Yes, it enhances cytotoxicity of fluoropyrimidines and antifolate drugs in preclinical models.
5. What is the molecular mechanism of Calcium Folinate?
It converts to CH2-THF intracellularly, cofactor for thymidylate synthase, stabilizes DNA synthesis, and modulates apoptotic responses.
6. Is 100 mg powder suitable for extended preclinical studies?
Yes, convenient packaging for dosing flexibility in vitro and in vivo.
7. Can Calcium Folinate support resistance mechanism studies?
Yes, it allows evaluation of transporter activity, TS expression, and folate-dependent drug resistance pathways.
8. Is Calcium Folinate supplied by a China chemical manufacturer?
Yes, sourced from verified factory suppliers with OEM and bulk production available.
9. How is Calcium Folinate verified for quality?
Each batch is validated using HPLC, MS, NMR, and supplied with a CoA.
10. Can it be used for DNA synthesis and cell-cycle studies?
Yes, widely used to study S-phase progression, checkpoint activation, and nucleotide biosynthesis.
11. Is it compatible with in vivo preclinical studies?
Yes, with appropriate formulation for animal research.
12. Are bulk orders available?
Yes, OEM and bulk supply are available.
13. Can Calcium Folinate powder be used for high-throughput screening?
Yes, powder form allows precise preparation and dosing for large-scale assays.
14. Does Calcium Folinate induce apoptosis alone?
Alone it has minimal cytotoxicity; apoptosis is typically observed in combination with antifolate or chemotherapy agents.
15. What applications are suitable for 100 mg Calcium Folinate powder?
Folate metabolism research, thymidylate synthase modulation, chemosensitization studies, DNA synthesis research, combination therapy evaluation, cytotoxicity assays, and cell-cycle analysis.
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