Description

DOTA-NOC (DOTA-Nal³-octreotide) is a synthetic, high-affinity peptide ligand targeting somatostatin receptor subtypes 2, 3, and 5. This tailored peptide, conjugated with the well-established macrocyclic chelator DOTA, enables highly efficient radiometal labeling, facilitating radiopeptide imaging and targeted radionuclide development. With a purity of 99.22% (HPLC), produced under stringent GMP protocols, DOTA-NOC ensures consistent performance across preclinical and translational research applications. It provides a reliable foundation for creating radiolabeled imaging agents and dendritic radionuclide-drug conjugates (RDCs), notably enhancing specificity and imaging clarity.

Product Specifications

Mechanism of Action & Research Applications

Mechanism of Action
DOTA-NOC binds with high affinity to somatostatin receptor subtypes 2, 3, and 5—affinities significantly higher than many traditional analogs. Radiometal labeling (e.g., with ^111In, ^90Y) yields radiopeptides that internalize rapidly within receptor-positive tumor cells and demonstrate superior tumor uptake compared to earlier agents. This includes several-fold higher internalization and retention, led by enhanced receptor engagement and favorable pharmacokinetics.

Research Applications

• Radiopeptide Imaging: Create imaging agents for PET or SPECT to visualize tumors overexpressing somatostatin receptors, particularly neuroendocrine tumors.

• Radiotherapy Development: Serve as a scaffold for creating therapeutic radionuclide conjugates capable of delivering cytotoxic payloads to receptor-positive tissues.

• Comparative Binding Studies: Benchmark against other DOTA-conjugated peptides (e.g., DOTA-TOC) to investigate receptor subtype selectivity and internalization kinetics.

• Pharmacokinetic Profiling: Study biodistribution, clearance, and targeting efficiency in animal models to optimize dosing and imaging timelines.

Development & Formulation Notes

Radiolabeling Preparation
Prepare DOTA-NOC solutions in an appropriate buffer (e.g., sodium acetate, pH 4.5–5.5) optimized for efficient chelation. Use trace-metal-free reagents. Radiolabeling typically employs Ga-68, In-111, Y-90, or Lu-177 under controlled temperature conditions. Perform purification and quality control via radio-HPLC, confirming radiochemical purity >95%.

Storage & Handling
Store lyophilized DOTA-NOC at –20 °C. Upon reconstitution, aliquot small volumes and store at –80 °C if needed to maintain stability. Avoid repeated freeze–thaw cycles.

Assay Design Guidance
In receptor binding assays, generate dose-response curves and measure internalization rates in receptor-expressing cell lines. For biodistribution studies, assess organ uptake, tumor targeting, and clearance profiles at multiple time points.

Disclaimer

DOTA-NOC is supplied strictly for laboratory research use only. It is not a pharmaceutical, diagnostic, or therapeutic product and must not be used for any human or veterinary applications. This product should be handled by trained personnel using appropriate biosafety protocols, including PPE. Disposal must comply with institutional and local regulations. All data referenced reflect preclinical research context; users are responsible for validating suitability and regulatory compliance for their experiments.

Packaging & Availability

DOTA-NOC is offered in these packaging formats:

• Standard Packs: 1 mg, 5 mg, 10 mg – ideal for academic or small-screening needs.

• Bulk/Wholesale: Multi-gram lots with batch reservation and continuity for extended programs.
  Each lot includes a Certificate of Analysis, detailing purity, identity, endotoxin levels, and appearance. Additional QC documentation is available for audit or regulatory purposes upon request.

Quality Assurance

Manufactured under GMP-compliant systems, each batch of DOTA-NOC undergoes thorough testing for identity (MS), purity (HPLC), sterility, endotoxin, and stability. Appearance and moisture content are documented. Radiolabeling compatibility is tested to ensure consistent performance. Custom QC reports are available to support regulatory submissions or institutional quality standards.

Custom Services

To support your specific research needs, we offer:

• Custom Aliquots & Labeling: Tailored packaging to align with your LIMS or ELN systems.

• Radiolabeling Protocol Support: Guidance on optimal labeling conditions for different radionuclides.

• Stability Study Design: Consultation for assessing storage and shipment stability under various conditions.

• Application-Specific Advising: Help with imaging, biodistribution, or radionuclide therapy experimental design.

Keywords

DOTA-NOC; DOTA-Nal3-octreotide; somatostatin receptor ligand; sstr2 sstr3 sstr5 agonist; radiometal labeling peptide; radiopeptide imaging; RDC synthesis peptide; PET imaging precursor; GMP peptide; CAS 619300-53-7; research-use-only.