Description

Product Description

PEG‑rhG‑CSF is a recombinant human granulocyte colony-stimulating factor (rhG‑CSF) that has been covalently conjugated with polyethylene glycol (PEG) to enhance its molecular stability and prolong its half-life for laboratory research. This PEGylated form is designed to maintain full biological activity while offering improved solubility, reduced immunogenicity, and extended circulation in experimental models.

The primary biological function of PEG‑rhG‑CSF is to bind to the G‑CSF receptor (G‑CSFR) on hematopoietic progenitor cells in bone marrow, activating intracellular signaling cascades that stimulate proliferation, differentiation, and maturation of neutrophilic granulocytes. Laboratory studies use PEG‑rhG‑CSF to model hematopoietic recovery, study cytokine-receptor interactions, and investigate signal transduction pathways including JAK/STAT, MAPK, and PI3K/AKT.

In vitro research demonstrates that PEGylation does not significantly alter receptor-binding affinity, but it enhances protein stability against enzymatic degradation, proteolysis, and aggregation. This property allows for more consistent dosing and extended incubation periods in experimental cell cultures.

Experimental applications of PEG‑rhG‑CSF include:

• Hematopoietic progenitor assays: Investigating cell proliferation, differentiation, and colony formation in bone marrow cultures.

• Immune modulation studies: Examining neutrophil expansion, chemotaxis, and functional activity in vitro and animal models.

• Pharmacokinetic modeling: Evaluating the effects of PEGylation on protein half-life, biodistribution, and receptor occupancy.

• Signal transduction research: Studying intracellular pathways activated by G‑CSFR engagement.

• Combination studies: Exploring synergistic effects with cytokines, growth factors, or experimental therapeutics in laboratory models.

PEG‑rhG‑CSF is supplied as a sterile lyophilized powder or solution, with ≥95% purity, suitable for laboratory preparation and in vitro or in vivo research. Each batch undergoes strict quality control, including protein characterization, PEGylation confirmation, and bioactivity assays. This ensures reproducibility, experimental accuracy, and reliable modeling of neutrophil biology.

Product Specifications

Mechanism of Action

PEG‑rhG‑CSF functions as a potent hematopoietic growth factor by binding to the G-CSF receptor on progenitor cells, initiating intracellular signaling pathways that drive neutrophil production and maturation.

Receptor Binding

PEG‑rhG‑CSF interacts with G-CSFR expressed on hematopoietic stem and progenitor cells. This high-affinity binding triggers receptor dimerization and conformational changes, activating downstream signaling cascades.

Signal Transduction

Activation of G-CSFR initiates JAK/STAT, MAPK, and PI3K/AKT pathways, promoting transcriptional programs that regulate cell proliferation, survival, and differentiation.

PEGylation Advantages

Covalent attachment of PEG chains improves protein solubility, prolongs half-life, reduces immunogenicity, and enhances stability against proteases. This allows extended exposure in experimental systems without significant loss of bioactivity.

In Vitro and In Vivo Research Applications

PEG‑rhG‑CSF is widely used in:

• Cell culture models to quantify neutrophil lineage expansion

• Hematopoietic colony-forming unit (CFU) assays

• Pharmacokinetic and pharmacodynamic modeling in animal studies

• Investigating signaling cross-talk and combination effects with other cytokines

  

Side Effects

In laboratory studies, PEG‑rhG‑CSF demonstrates minimal cytotoxicity at research concentrations. Off-target effects are rare but may include transient changes in cell viability or proliferation at high experimental doses. Researchers should monitor experimental conditions carefully, including protein concentration, incubation time, and culture environment.

PEG‑rhG‑CSF is not intended for human or veterinary administration outside of laboratory research.

Keywords

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Shipping Guarantee

All shipments of PEG‑rhG‑CSF are handled using validated cold-chain logistics to preserve protein integrity. Each package is sealed in sterile, moisture-proof containers with secondary protective wrapping and continuous temperature monitoring. Products are shipped via express international couriers with full tracking and insurance coverage, ensuring delivery without degradation or loss of bioactivity.

Trade Assurance

We guarantee the authenticity and high purity (≥95%) of PEG‑rhG‑CSF. Each batch undergoes stringent quality control, including protein characterization, PEGylation confirmation, and bioactivity assays. A Certificate of Analysis (CoA) is provided for each batch. Our trade assurance policy ensures replacement or refund for any deviation from listed specifications, including purity, PEGylation integrity, or stability.

Payment Support

We provide secure and flexible payment options for global transactions, supporting PayPal, major credit cards (Visa, MasterCard, American Express), telegraphic transfer (T/T), and cryptocurrencies (USDT, Bitcoin, Ethereum). All transactions are encrypted and verified, ensuring fund security and confidentiality.

Disclaimer

All PEG‑rhG‑CSF products are intended for laboratory research use only. They are not drugs, medical devices, or diagnostics, and should not be administered to humans or animals. Researchers must follow institutional biosafety and chemical safety guidelines. Information provided is for scientific reference only and does not imply therapeutic efficacy, safety, or regulatory approval.