Description

Pezadeftide is a plant-derived antifungal peptide renowned for its rapid and potent fungicidal action. At a molecular sequence length of 51 amino acids and multiple disulfide bridges, this peptide combines structural stability with biological specificity. Its primary antifungal mechanism involves penetrating fungal cell walls, then triggering mitochondrial membrane hyperpolarization, which initiates a cascade of reactive oxygen species (ROS) production, cellular membrane permeabilization, leading to swift fungal cell death.

The efficacy of Pezadeftide spans multiple fungal pathogens, including Candida albicans, Trichophyton species, and Microsporum. In ex vivo and early clinical studies for fungal infections such as onychomycosis (nail fungal infection), it demonstrated promising mycological cure rates.

Manufactured under strict GMP standards, Pezadeftide is supplied at 99.8% purity (analytical), providing reliability for research and preclinical model development. It is supplied as a lyophilized powder and available in both bulk (wholesale) and smaller quantities (retail) to support any scale of laboratory need.

Note: Intended exclusively for laboratory research use—not for human, veterinary, or diagnostic usage.

Product Specifications

Mechanism of Action & Research Applications

Pezadeftide demonstrates a distinct and rapid antifungal mechanism:

1. Cell Entry and Targeting
   The peptide accumulates at the fungal cell wall and subsequently enters the cytoplasm, as shown by fluorescence microscopy in studies with Trichophyton rubrum and C. albicans.

2. Mitochondrial Hyperpolarization
   Once inside, Pezadeftide induces a sharp increase in mitochondrial membrane potential, exceeding that observed in control cells and even compared to known depolarizers like CCCP.

3. ROS Generation
   Hyperpolarization initiates excessive ROS production, confirmed via the DHR123 fluorescent probe. In C. albicans, up to 74% of cells exhibited elevated ROS levels at standard peptide concentrations.

4. Cell Membrane Disruption
   The peptide-mediated mechanisms culminate in plasma membrane permeabilization, demonstrated by uptake of nucleic acid stains like SYTOX green—indicating fungal cell death.

Research Applications

• Antifungal Mechanism Studies – Investigate mitochondrial and oxidative pathways in pathogen response.

• Drug Resistance Models – Evaluate the effectiveness of novel antifungal compounds or combinational strategies.

• Mitochondrial Hyperpolarization – Model mitochondrial dynamics and ROS-mediated cell death in fungal systems.

• Preclinical Trial Design – Support early development studies targeting fungal infections and drug delivery systems.

  

Side Effects (For Reference in Models)

Though intended for fungal systems, model studies and peptide screening have highlighted the following potential research observations:

• Selective Fungal Toxicity – Pezadeftide exhibits negligible toxicity toward human and bacterial cells (IC50 and MIC values > 50 ?g/mL), indicating strong specificity.

• Intracellular ROS Surge – Intense ROS production may complicate assays sensitive to oxidative stress.

• Peptide Instability in Solution – Long-term storage in solution may reduce activity; recommended to store as powder.

These observations are meant for experimental design considerations and are not indicative of safety in clinical contexts.

Disclaimer

Pezadeftide is supplied strictly for laboratory research use. It is not intended for human or veterinary use, nor for diagnostic or therapeutic purposes. Researchers must adopt appropriate safety protocols in handling and experimentation.

Keywords

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