Description

Selcopintide Acetate (also known as Cpne7-DP) is a precisely engineered 10-residue synthetic peptide corresponding to fragment 344–353 of the human CPNE7 protein. CPNE7 (copine-7) plays a critical role in odontoblastic differentiation and dentin formation. The selected fragment, when synthesized and administered exogenously, recapitulates the signaling cascade of full-length CPNE7, resulting in significant upregulation of odontoblast markers, including DSPP (dentin sialophosphoprotein) and Nestin, which are essential for odontoblast function and dentin matrix deposition.

In experimental dentin defect models, Selcopintide Acetate has demonstrated robust ability to drive dentin regeneration. Defects treated with this peptide show newly formed hard tissue with structural and histological characteristics of true dentin, including tubular organization and mineral density consistent with native dentin. This makes Selcopintide an invaluable research tool in dental tissue engineering, regenerative dentistry, and developmental biology studies involving tooth repair mechanisms.

Our formulation is produced under GMP-compliant conditions, with high purity (99.83%) verified by analytical methods. The peptide is supplied as a stable lyophilized powder, suitable for dissolution and use in in vitro cultures, ex vivo organ cultures, and in vivo application in animal dentin injury models. Each batch includes a Certificate of Analysis (CoA) for identity, purity, and peptide integrity, facilitating study reproducibility and regulatory compliance in academically aligned settings.

Selcopintide Acetate Product Specifications

Selcopintide Acetate Mechanism of Action & Research Applications

Mechanism of Action

Selcopintide Acetate mimics the biologically active fragment of human CPNE7, engaging cellular pathways that drive odontoblastic differentiation. By activating transcriptional programs, it specifically upregulates DSPP—a critical noncollagenous protein in dentin mineralization—and Nestin, a marker of odontoblast progenitors. The result is enhanced deposition of mineralized dentin matrix with structural fidelity resembling natural dental tissue.

Research Applications

• Dental Tissue Engineering: Used to induce reparative dentin formation in tooth injury models, either in ex vivo organ cultures or in vivo rodent models. Key readouts include histology (dentin tubules), mineral density, and expression of odontoblastic markers.

• Stem Cell Differentiation: Applied to dental pulp stem cells or mesenchymal progenitors to explore signal pathways that drive odontoblast lineage progression.

• Developmental Biology: Provides a tool to study CPNE7’s role in tooth morphogenesis and to identify downstream effectors of dentinogenic signaling.

• Biomaterials Research: Can be incorporated into scaffold systems, hydrogels, or nanoparticle-based carriers to deliver contextual signals for dental imprint regeneration.

• Regenerative Dentistry Models: Enables exploration of dose–effect relationships, temporal kinetics of regeneration, and synergy with other growth factors or signaling modulators.

Selcopintide Acetate Development & Formulation Notes

Handling & Reconstitution

• Reconstitute lyophilized peptide in sterile buffer (e.g., PBS) to desired stock concentration (common: 1 mg/mL).

• Use low-binding tubes and prepare aliquots to avoid repeated freeze–thaw cycles.

• Store reconstituted solutions short-term at 4–8 °C, or longer at –80 °C.

Assay Design

• Common in vitro assays include odontoblast marker induction, mineralization nodules, and calcium deposition assays. Compare baseline vs. Selcopintide-treated cultures.

• In ex vivo tooth slice or root canal defect models, histological analysis may include measurements of newly formed dentin thickness, tubular density, and marker expression by immunostaining.

Formulation Strategies

• Combine with hydrogels or collagen matrices for in situ delivery in root canal or pulp chamber models.

• Evaluate release kinetics and peptide stability within delivery vehicles over experimental time frames (e.g., 1–4 weeks).

Analytical Validation

• Confirm peptide integrity in reconstituted solution via HPLC retention and mass verification for long-duration experiments.

• Assess peptide concentration post-formulation by UV or peptide-specific assays to ensure dosing accuracy.

  

Disclaimer

Selcopintide Acetate is for laboratory research use only. It is not intended for therapeutic, diagnostic, or clinical applications. Users must follow institutional regulations and biosafety protocols. The efficacy statements mentioned here apply solely to preclinical or in vitro research contexts and should not be construed as medical advice.

Keywords

Selcopintide Acetate; Cpne7-DP; odontoblast differentiation peptide; dentin regeneration peptide; CPNE7 fragment; DSPP activator; Nestin upregulation; dental tissue engineering; regenerative dentistry; GMP-grade peptide; tooth repair research peptide.