Description

Product Description

Tadalafil (CAS 171596-29-5) is a high-purity chemical reagent designed specifically for in vitro mechanistic studies and molecular pathway research. Manufactured under strict quality control, it provides researchers with a consistent and reliable compound to investigate enzyme modulation, receptor interactions, and intracellular signaling mechanisms in controlled laboratory environments. Each batch is thoroughly analyzed and verified to ensure purity, identity, and reproducibility, making it a dependable reagent for mechanistic research applications.

Supplied as a stable solid powder, Tadalafil supports a wide variety of biochemical, enzymatic, and molecular assays. Its well-defined chemical structure allows precise formulation of stock and working solutions, enabling researchers to perform quantitative studies of enzyme inhibition, binding kinetics, and molecular interaction dynamics. This makes it particularly valuable for studies of structure-function relationships and mechanistic pathway modulation.

In mechanistic in vitro research, Tadalafil is commonly employed as a model phosphodiesterase (PDE) inhibitor. Its selective inhibition of specific PDE isoforms allows detailed examination of cyclic nucleotide regulation, signal transduction events, and downstream molecular responses. Researchers can utilize Tadalafil to probe enzyme activity, receptor-mediated signaling, and molecular cross-talk in controlled assay systems, supporting a deep understanding of cellular signaling architecture.

Tadalafil is also suitable for integration into high-throughput screening platforms, including plate-based enzymatic assays and receptor-ligand interaction studies. Its chemical stability and reproducibility across batches enable comparative mechanistic analyses, dose-response studies, and temporal signaling investigations, facilitating robust experimental design.

Factory-direct manufacturing ensures consistent batch quality, analytical verification, and cost-effective bulk supply, allowing laboratories to maintain long-term mechanistic research workflows. Each shipment is accompanied by a Certificate of Analysis (COA) documenting purity, chemical identity, and batch-specific verification, ensuring traceability and reliability.

Strictly intended for in vitro laboratory research, Tadalafil allows precise investigation of enzyme signaling, molecular interactions, and mechanistic pathway regulation. Its high purity, reproducible performance, and availability in scalable quantities make it a versatile tool for researchers exploring PDE-related signaling networks and molecular mechanisms in mechanistic studies.

Product Specifications

Mechanism of Action

Tadalafil functions as a selective phosphodiesterase (PDE) inhibitor, with a primary target of the PDE5 isoform. In controlled in vitro mechanistic studies, it modulates cGMP degradation, resulting in increased intracellular cyclic nucleotide concentrations. This property enables researchers to investigate enzyme kinetics, receptor-mediated signaling, and downstream molecular effects under reproducible laboratory conditions. By precisely regulating PDE activity, Tadalafil allows for detailed mechanistic studies of signal transduction pathways and the molecular networks associated with cyclic nucleotide signaling.

In enzyme inhibition assays, Tadalafil binds selectively to the active site of PDE5, stabilizing the enzyme in an inactive conformation. This inhibition reduces the hydrolysis of cyclic guanosine monophosphate (cGMP), thereby altering intracellular second messenger dynamics. Researchers can use this controlled modulation to explore enzyme-substrate interactions, allosteric effects, and inhibitory kinetics, providing quantitative insights into enzyme regulation and pathway specificity.

Tadalafil also serves as a valuable tool for mechanistic exploration of signaling cascades in reconstituted systems. By modulating cGMP levels, researchers can monitor downstream effectors, molecular crosstalk, and feedback regulation within complex networks. This facilitates a deeper understanding of cellular signal propagation, enzyme interplay, and pathway hierarchy, which is critical for elucidating mechanistic principles in vitro.

Its chemical stability and high purity ensure consistent activity across experimental replicates, enabling researchers to perform comparative mechanistic analyses, dose-response studies, and temporal signaling assessments. Tadalafil is compatible with a variety of assay formats, including cell-free enzyme systems, microplate-based receptor-ligand interaction studies, and high-throughput screening platforms, supporting scalable experimental designs.

Furthermore, Tadalafil-mediated mechanistic data can be integrated into computational models and systems biology frameworks. Quantitative measurements of PDE inhibition, cGMP dynamics, and pathway responses can inform predictive simulations, network modeling, and pathway enrichment analyses, enabling researchers to visualize molecular interactions and signaling networks in silico. Such integration enhances the interpretive value of mechanistic in vitro studies and facilitates the development of hypothesis-driven research.

Structural diagram or schematic :

Overall, Tadalafil provides a robust and versatile reagent for laboratory research focused on enzyme modulation, cyclic nucleotide regulation, and mechanistic signaling studies. Its reproducible inhibition profile, compatibility with multiple assay formats, and integration potential with computational analyses make it an indispensable tool for advanced in vitro mechanistic investigations.

Applications

Tadalafil is widely utilized in in vitro mechanistic and molecular studies, providing a versatile reagent for exploring enzyme modulation, signal transduction, and molecular interactions. Its high purity and reproducibility make it suitable for a variety of controlled laboratory experiments.

One primary application is in enzyme inhibition studies, particularly targeting phosphodiesterase (PDE) isoforms. Researchers can evaluate PDE activity, inhibition potency, and kinetic profiles, supporting mechanistic insights into cyclic nucleotide regulation and downstream signaling effects.

Tadalafil is also applied in signal transduction research, where modulation of cGMP-mediated pathways enables investigation of receptor-mediated signaling, second messenger dynamics, and pathway crosstalk. By stabilizing intracellular cyclic nucleotide levels, it allows researchers to probe molecular interactions and pathway regulation in defined experimental conditions.

In structure-function analyses, Tadalafil serves as a benchmark inhibitor for comparative studies of chemical analogs or derivatives, facilitating mechanistic evaluation of structure-activity relationships. Its consistent performance across batches ensures reliable interpretation of experimental results.

Additionally, Tadalafil is compatible with high-throughput assay platforms, including microplate-based enzyme activity measurements, receptor-ligand interaction studies, and automated screening systems. This versatility supports scalable experiments, comparative mechanistic analyses, and reproducible temporal or concentration-dependent studies.

By integrating Tadalafil into controlled in vitro research workflows, scientists can gain detailed understanding of enzyme regulation, molecular interaction networks, and signal transduction dynamics, providing a robust foundation for mechanistic and molecular-level research.

Research Models

Tadalafil is compatible with a variety of in vitro research models designed to explore enzyme regulation, signal transduction, and molecular interactions. Its high purity and reproducible performance make it suitable for mechanistic studies requiring precise quantitative measurements.

One commonly used model is cell-free enzyme assays, where Tadalafil is applied to examine phosphodiesterase (PDE) activity, inhibition kinetics, and isoform selectivity. These assays allow researchers to directly quantify enzyme modulation and determine concentration-dependent effects without cellular complexity.

Tadalafil is also utilized in reconstituted signaling systems, such as isolated cGMP-mediated pathways, to investigate downstream signaling events, feedback loops, and molecular network interactions. These models enable detailed mechanistic analysis of intracellular signal propagation under controlled conditions.

For studies focusing on molecular interactions, Tadalafil can be incorporated into protein-protein interaction platforms to probe enzyme-substrate binding, allosteric regulation, and pathway modulation. Its chemical stability ensures consistent activity measurements across experimental replicates.

High-throughput applications are supported in microplate-based assays and automated screening systems, allowing researchers to evaluate kinetic parameters, inhibitor potency, and pathway dynamics across multiple conditions simultaneously. These models facilitate comparative analyses and mechanistic simulations for peptide or enzyme-targeted research.

By using Tadalafil in these research models, laboratories can achieve reliable, reproducible, and interpretable data on enzyme behavior, signal transduction, and mechanistic pathway regulation, providing a robust platform for advanced in vitro studies.

Experimental Design Considerations

When designing experiments using Tadalafil, careful planning is essential to ensure reproducible and interpretable results. Researchers should prepare accurate stock and working solutions using validated laboratory-grade solvents, such as DMSO or ethanol, while maintaining appropriate concentration ranges for the specific PDE isoforms or signaling pathways under study.

It is recommended to include control groups and replicates in all assays to distinguish compound-specific effects from background noise. Negative controls without Tadalafil, as well as vehicle controls containing only the solvent, are critical for proper baseline measurement.

The incubation time, temperature, and assay conditions should be optimized based on the enzyme or signaling system being investigated. Consistent environmental parameters, including pH and ionic strength, are crucial to minimize variability and preserve enzymatic activity.

Tadalafil’s stability and solubility should be considered when planning experiments. Avoid repeated freeze-thaw cycles, and aliquot stock solutions for single-use to prevent degradation. Mixing should be gentle to maintain chemical integrity and prevent aggregation.

For kinetic or mechanistic studies, concentration-response curves are highly recommended. This allows accurate determination of enzyme inhibition, IC50 values, or kinetic parameters, providing quantitative insights into PDE regulation and signaling dynamics.

Finally, documentation of batch numbers, preparation protocols, and assay parameters is essential for traceability and reproducibility. Detailed record-keeping supports comparison between experiments and ensures robust mechanistic conclusions.

Laboratory Safety & Handling Guidelines

Tadalafil is a high-purity chemical reagent intended for in vitro mechanistic and molecular research. Proper handling and storage are critical to maintain compound integrity and ensure laboratory safety. All personnel working with Tadalafil should follow standard laboratory safety protocols, including the use of appropriate personal protective equipment (PPE), such as gloves, lab coats, and safety goggles. Handling should occur in a clean, controlled environment, such as a fume hood or biosafety cabinet, to prevent contamination and minimize accidental exposure.

The solid powder should be protected from moisture, light, and temperature extremes. Store Tadalafil at –20 °C in a dry, dark location. Repeated freeze-thaw cycles should be avoided, as they may compromise chemical stability and affect reproducibility in mechanistic studies. Aliquoting the compound into smaller portions for single-use is recommended to reduce exposure and preserve activity over long-term storage.

When preparing stock and working solutions, use validated laboratory-grade solvents such as DMSO or ethanol, ensuring complete dissolution while maintaining chemical integrity. Gentle mixing is preferred to avoid compound aggregation or degradation. Solutions should be labeled clearly with compound name, concentration, preparation date, and storage conditions to maintain traceability and reproducibility in experiments. Avoid prolonged exposure to ambient conditions during preparation and handling.

All laboratory personnel should be trained in proper handling, storage, and emergency procedures specific to Tadalafil. Safety data sheets (SDS) and institutional guidelines should be readily accessible. In case of accidental spills, clean-up should be performed immediately using appropriate materials, and contaminated surfaces should be decontaminated according to laboratory protocols. Avoid inhalation, ingestion, or direct contact with the skin or eyes, and wash hands thoroughly after handling.

Waste containing Tadalafil should be collected and disposed of according to institutional and local laboratory regulations. This includes used solutions, contaminated consumables, and packaging materials. Proper labeling and segregation of waste prevent accidental exposure and ensure compliance with environmental and safety standards.

Documentation is essential for traceability and reproducibility. Record batch numbers, storage conditions, preparation protocols, and assay parameters meticulously. Such records enable consistent experimental results, support quality assurance, and facilitate verification of mechanistic study outcomes.

Tadalafil is compatible with high-throughput screening, microplate-based enzymatic assays, and receptor-ligand interaction studies, but careful attention to handling, solvent selection, and environmental control is required to maintain experimental integrity. Consistency in temperature, pH, ionic strength, and incubation time across experiments is essential to obtain reliable data.

By adhering to these comprehensive laboratory safety and handling guidelines, researchers can ensure that Tadalafil is used safely, efficiently, and reproducibly in all in vitro mechanistic studies, minimizing risk and maximizing the quality and reliability of experimental data.

Integration with Multi-Omic & Computational Studies

Tadalafil-generated in vitro data can be effectively integrated with multi-omic analyses to provide comprehensive insights into molecular mechanisms. For example, proteomic and phosphoproteomic datasets can be combined with Tadalafil-mediated PDE inhibition studies to map downstream signaling networks and post-translational modifications in a mechanistic context.

Integration with transcriptomic and metabolomic data enables researchers to correlate enzyme activity modulation with gene expression patterns and metabolite dynamics, supporting a holistic understanding of intracellular signaling pathways. These approaches help uncover cross-talk between pathways and compensatory mechanisms in response to PDE inhibition.

Tadalafil data can also be incorporated into computational modeling frameworks to simulate enzyme kinetics, pathway dynamics, and signaling fluxes. Quantitative modeling supports the design of mechanistic experiments, prediction of pathway responses, and identification of critical nodes within molecular networks.

Furthermore, multi-parameter datasets derived from Tadalafil studies can be used to perform network analysis, pathway enrichment, and predictive simulations, enabling researchers to visualize mechanistic interactions and generate hypotheses for further experimental validation. This integration enhances the interpretive value of in vitro mechanistic studies, supporting robust and reproducible laboratory research.

Things to Note

• Tadalafil is intended strictly for in vitro laboratory research. It is not for human, animal, or clinical applications.

• Protect the compound from moisture, light, and extreme temperatures to preserve chemical integrity. Store at –20 °C in a dry, dark environment, and avoid repeated freeze-thaw cycles.

• Prepare accurate stock and working solutions using validated solvents such as DMSO or ethanol. Aliquot stock solutions for single-use to maintain reproducibility and chemical stability.

• Include proper experimental controls in all assays, including negative and vehicle controls, to distinguish specific effects from background noise.

• Maintain detailed records of batch numbers, preparation protocols, and assay parameters to ensure traceability and reliable mechanistic data.

• Use appropriate personal protective equipment (PPE), such as gloves, lab coat, and eye protection, when handling the powder or solutions.

• Dispose of Tadalafil-containing waste according to institutional and local laboratory safety guidelines.

• Ensure consistent experimental conditions (pH, temperature, incubation time, ionic strength) to minimize variability in mechanistic studies.

Tadalafil is compatible with high-throughput platforms and comparative studies, but experimental design should account for its stability and solubility limitations.

Keywords

Tadalafil, PDE5 inhibitor, in vitro research, enzyme modulation, mechanistic study, molecular signaling, cyclic GMP regulation, phosphodiesterase assay, high-purity reagent, laboratory research chemical, mechanistic pathway analysis, receptor-ligand interaction, enzyme kinetics, high-throughput screening, molecular mechanism exploration, signal transduction, cellular pathway study, structure-function relationship, enzyme inhibition, pharmacological research tool.

Shipping Guarantee

• Secure laboratory-grade packaging ensures Tadalafil remains stable and uncontaminated during transit. Temperature-controlled logistics maintain chemical integrity, preventing degradation from heat or moisture. Global delivery options guarantee reliable shipment to research laboratories worldwide. Tracking and handling protocols are provided to ensure compounds arrive safely and promptly for experimental use.

Trade Assurance

• Factory-direct manufacturing guarantees consistent batch quality and reproducible performance across experiments. Each shipment is accompanied by a Certificate of Analysis (COA) confirming purity, chemical identity, and analytical validation. Bulk and wholesale supply options are available to support high-throughput mechanistic research and long-term experimental workflows. Traceable production ensures researchers can maintain reproducible and reliable data collection.

Payment Support

• Credit Card: Visa / MasterCard / AMEX

• Telegraphic Transfer (T/T)

• Cryptocurrency: BTC, ETH, supported digital assets

• All transactions are processed through encrypted, secure channels.

• Flexible options facilitate international laboratory procurement.

Disclaimer

• Tadalafil is intended strictly for laboratory research use only and is not for human, animal, or clinical applications. All experimental use must comply with institutional laboratory safety protocols and regulations. Researchers are responsible for proper handling, storage, and disposal in accordance with laboratory safety guidelines. Use of this product in any unauthorized context is strictly prohibited and outside the scope of intended research applications.

References

1. Selleck Chemicals — Tadalafil: https://www.selleckchem.com/product/tadalafil.html

2. PubChem — Tadalafil: https://pubchem.ncbi.nlm.nih.gov/compound/Tadalafil

3. ChemSpider — Tadalafil: http://www.chemspider.com/Chemical-Structure.173795.html

4. TargetMol — Tadalafil: https://www.targetmol.com/catalog/Tadalafil.html

5. NCBI PubMed — PDE inhibitors mechanistic study: https://pubmed.ncbi.nlm.nih.gov/31436004/

6. Sigma-Aldrich — Tadalafil research reagent: https://www.sigmaaldrich.com/catalog/product/sigma/t0897

7. PeptideLibrary.com — Tadalafil in vitro studies: https://www.peptidelibrary.com/product/tadalafil

8. DrugBank — Tadalafil: https://go.drugbank.com/drugs/DB00203

9. MedChemExpress — Tadalafil research: https://www.medchemexpress.com/Tadalafil.html

10. ChemicalBook — Tadalafil CAS 171596-29-5: https://www.chemicalbook.com/ProductChemicalPropertiesCB9721321_EN.htm