Urofollitropin (CAS 97048-13-0) | GMP Supplier & Manufacturer

描述

Product Description

Background

Urofollitropin is a highly purified form of follicle-stimulating hormone (FSH), extracted from the urine of postmenopausal women. As a critical glycoprotein hormone, FSH regulates reproductive processes in mammals, particularly follicle development in females and spermatogenesis in males.

The urinary-derived version of FSH, such as Urofollitropin, has been extensively studied for its role in controlled ovarian hyperstimulation, follicle recruitment, and maturation. It contains minimal urinary proteins compared to crude extracts, making it ideal for consistent and reproducible experimental results.

Chemical Nature and Source

Urofollitropin is a heterodimeric glycoprotein consisting of two non-covalently linked subunits:

• Alpha subunit: common to all glycoprotein hormones (LH, TSH, hCG).

• Beta subunit: confers biological specificity to FSH by binding selectively to FSH receptors.

Due to its glycosylation patterns, the molecular weight of Urofollitropin ranges from ~30–35 kDa.

The extraction process from human menopausal urine involves multiple purification steps, resulting in a product with high specific activity and reduced non-target urinary protein content.

Research Significance

Urofollitropin has profound applications in reproductive biology and endocrinology research. By binding to FSH receptors in ovarian granulosa cells, it promotes follicular growth, estrogen production, and oocyte maturation. In experimental settings, it allows scientists to model hormonal regulation of fertility, ovarian physiology, and mechanisms of controlled follicle stimulation.

Compared to recombinant FSH, Urofollitropin offers unique advantages in terms of cost-effectiveness, historical research validation, and availability in bulk supply.

Comparative Advantage

• High Purity: Significantly reduced non-hormonal proteins.

• Biological Activity: Mimics endogenous FSH effects on follicular maturation.

• Research Reliability: Consistent response in in vitro and in vivo models.

• GMP-Grade Production: Ensures reproducibility in laboratory studies.

Product Specifications

Mechanism of Action & Research Applications

Mechanism of Action

Urofollitropin exerts its biological function by binding to FSH receptors (FSHR), which are G protein-coupled receptors expressed on granulosa cells of the ovary and Sertoli cells of the testis. Upon receptor binding, it activates the adenylate cyclase–cAMP–PKA signaling cascade, leading to transcriptional regulation of genes involved in folliculogenesis and spermatogenesis.

• In Ovarian Cells: Stimulates aromatase activity, promoting estrogen biosynthesis.

• In Follicular Development: Supports granulosa cell proliferation and oocyte maturation.

• In Testicular Models: Enhances Sertoli cell support of spermatogenesis.

Research Applications

1. Ovarian Biology Research – Study of follicle recruitment, growth, and maturation.

2. Endocrine Regulation Studies – Exploration of gonadotropin signaling pathways.

3. Fertility Research – Investigating hormonal control of ovulation and assisted reproduction.

4. Translational Applications – Preclinical models exploring controlled ovarian stimulation.

5. Comparative Studies – Benchmarking Urofollitropin against recombinant FSH analogues.

By serving as a reproducible and GMP-compliant FSH source, Urofollitropin provides scientists with a reliable tool for reproductive biology investigations.

Side Effects (For Reference in Research Models)

In experimental research, Urofollitropin has demonstrated biological activity consistent with endogenous FSH. Reported side effects (in animal and preclinical models) include:

• Ovarian Hyperstimulation: Dose-dependent follicular enlargement and cyst formation.

• Hormonal Imbalance: Excessive estrogen levels leading to systemic effects.

• Injection Site Reactions: Local irritation in in vivo delivery studies.

• Variability in Response: Differences in activity due to species-specific receptor expression.

• Protein Aggregation Risk: Improper storage or repeated freeze-thaw cycles may affect stability.

These observations highlight the importance of carefully controlled dosing, proper storage, and methodical study design in research models.

Disclaimer

For laboratory research use only. Not for human or veterinary use.

Keywords

• Urofollitropin CAS 97048-13-0

• Urofollitropin GMP Supplier

• Urinary-derived FSH protein extract

• Follicle-stimulating hormone research

• Urofollitropin peptide hormone

• Reproductive biology research reagent

• Ovarian follicle maturation study

• Gonadotropin hormone analog research

• Endocrine signaling peptide laboratory use

• Urofollitropin high purity lyophilized powder