Description

APP-018 (also known as D-4F) is a synthetic peptide consisting of 18 D-amino acids that mimics the anti-inflammatory and atheroprotective properties of apolipoprotein A-I (apoA-I). With 99.30% purity (HPLC), it has significant potential in the study of cardiovascular diseases and the regulation of high-density lipoprotein (HDL) function.

HDL plays a critical role in removing cholesterol from arterial walls and protecting against atherosclerosis. APP-018 mimics the functional properties of apoA-I by improving the anti-inflammatory effects of HDL. This makes it a valuable tool for researching the mechanisms of cardiovascular diseases, especially atherosclerosis and endothelial dysfunction. APP-018 offers a potent method for enhancing HDL functionality in experimental settings.

Produced under GMP-compliant manufacturing standards, APP-018 ensures high purity and reproducibility, making it suitable for sensitive laboratory experiments. It is supplied as a lyophilized powder, easy to reconstitute for use in various in vitro and in vivo research models.

Product Specifications

Mechanism of Action & Research Applications

Mechanism of Action
APP-018 mimics apolipoprotein A-I (apoA-I), a protein that plays a crucial role in modulating the anti-inflammatory properties of high-density lipoprotein (HDL). By improving HDL functionality, APP-018 enhances the ability of HDL to remove cholesterol from arterial walls and suppress inflammation. This action helps to reduce the risk of atherosclerosis and improve overall cardiovascular health. APP-018 achieves this by mimicking the structural and functional aspects of apoA-I, making it a useful tool in studying the mechanisms of cardiovascular diseases, particularly those related to lipid metabolism and endothelial dysfunction.

Research Applications (Illustrative Use Cases)

• Cardiovascular Disease Research: APP-018 can be used to study the role of HDL in the prevention of atherosclerosis and endothelial dysfunction. Researchers can assess the effects of this peptide on HDL-mediated cholesterol efflux and inflammation in cardiovascular models.

• Atherosclerosis Studies: APP-018 helps to understand how enhancing HDL functionality can prevent the development of atherosclerotic plaques. It can be used in both in vitro and in vivo studies.

• HDL Functionality: As a mimetic of apoA-I, APP-018 is valuable for evaluating the effects of HDL on inflammatory cytokines and its potential therapeutic application in cardiovascular diseases.

Development & Formulation Notes

Reconstitution & Handling
For use in experiments, reconstitute APP-018 in sterile water or PBS. For long-term storage, freeze reconstituted solutions at –20 °C or in aliquots to avoid freeze-thaw cycles. Ensure that sterility is maintained during reconstitution, and follow appropriate biosafety protocols for handling peptides.

Assay Design
When using APP-018 in assays, it is recommended to perform dose-response experiments to determine the optimal concentration for your research model. Depending on the research focus, it can be used in cell culture systems to study its effects on cholesterol efflux, inflammation, and endothelial function.

Disclaimer

APP-018 is intended for laboratory research use only and is not a pharmaceutical, medical device, or diagnostic product. It is not approved for human or veterinary use, and it is not intended for clinical or therapeutic applications. Researchers are responsible for determining the suitability of this product for their specific studies and for complying with all applicable safety regulations and guidelines.

Packaging & Availability

• Standard Packs: Available in 1 mg, 5 mg, and 10 mg for smaller-scale research.

• Bulk/Wholesale: Multi-gram quantities available for larger studies or institutions with ongoing research needs.
  Each lot is shipped with a Certificate of Analysis (CoA) detailing product purity, identity, and endotoxin levels.

Quality Assurance

APP-018 is manufactured in a GMP-compliant facility, ensuring consistent quality and reliability. The peptide undergoes rigorous testing for identity (MS), purity (HPLC), endotoxin levels, and other quality parameters. Additional documentation is available upon request for research or regulatory purposes.

Custom Services

We offer several custom services to support your research, including:

• Custom Aliquots & Labeling: Tailored packaging and labeling for specific needs or to align with your lab’s protocols.

• Peptide Synthesis: Custom synthesis of related peptides for your research applications.

• Stability Studies: Design and conduct studies to evaluate the stability of APP-018 under various storage conditions.

Keywords

APP-018; D-4F; Apolipoprotein A-I mimetic peptide; HDL functionality research; cardiovascular disease research; atherosclerosis studies; peptide for inflammation research; GMP peptide; CAS 452782-06-8; research-use-only peptide.